Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF-256-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human beta-NGF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human beta-NGF in direct ELISAs and Western blots. In direct ELISAs, greater than 50% cross-reactivity with recombinant mouse beta-NGF and recombinant rat beta-NGF is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
CAA36832
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane.
Nerve growth factor and artemin are paracrine mediators of pancreatic neuropathy in pancreatic adenocarcinoma.
Long-term delivery of nerve growth factor by encapsulated cell biodelivery in the Göttingen minipig basal forebrain.
Atropine-enhanced, antigen challenge-induced airway hyperreactivity in guinea pigs is mediated by eosinophils and nerve growth factor.
Dong Y, Kanda A, Noda K, Saito W, Ishida S
Scientific reports 2017 Mar 9;7:44266
Scientific reports 2017 Mar 9;7:44266
Nerve growth factor and artemin are paracrine mediators of pancreatic neuropathy in pancreatic adenocarcinoma.
Ceyhan GO, Schäfer KH, Kerscher AG, Rauch U, Demir IE, Kadihasanoglu M, Böhm C, Müller MW, Büchler MW, Giese NA, Erkan M, Friess H
Annals of surgery 2010 May;251(5):923-31
Annals of surgery 2010 May;251(5):923-31
Long-term delivery of nerve growth factor by encapsulated cell biodelivery in the Göttingen minipig basal forebrain.
Fjord-Larsen L, Kusk P, Tornøe J, Juliusson B, Torp M, Bjarkam CR, Nielsen MS, Handberg A, Sørensen JC, Wahlberg LU
Molecular therapy : the journal of the American Society of Gene Therapy 2010 Dec;18(12):2164-72
Molecular therapy : the journal of the American Society of Gene Therapy 2010 Dec;18(12):2164-72
Atropine-enhanced, antigen challenge-induced airway hyperreactivity in guinea pigs is mediated by eosinophils and nerve growth factor.
Verbout NG, Jacoby DB, Gleich GJ, Fryer AD
American journal of physiology. Lung cellular and molecular physiology 2009 Aug;297(2):L228-37
American journal of physiology. Lung cellular and molecular physiology 2009 Aug;297(2):L228-37
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- beta-NGF in Human Brain. beta-NGF was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human beta-NGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-256-NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to axons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by beta-NGF and Neutralization by Human beta-NGF Antibody. Recombinant Human beta-NGF (Catalog # 256-GF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human beta-NGF (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human beta-NGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-256-NA). The ND50 is typically 0.02-0.08 µg/mL.