HPA025235

antibody from Atlas Antibodies

Targeting: VANGL1 STB2

Provider product page for HPA025235

Western blot

Immunocytochemistry

Antibody data

Antibody Data
Antigen structure
References [16]
Comments [0]
Validations
Western blot [2]

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Product number: HPA025235 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA025235, RRID:AB_1858718
Product name: Anti-VANGL1
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human VANGL1, Gene description: VANGL planar cell polarity protein 1, Alternative Gene Names: STB2, Validated applications: ICC, WB, Uniprot ID: Q8TAA9, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.2 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Handling: The antibody solution should be gently mixed before use.

VANGL1 protein structure - HPA025235 shown in red.

Submitted references Junctional Adhesion Molecule 3 Expression in the Mouse Airway Epithelium Is Linked to Multiciliated Cells

Mateos-Quiros C, Garrido-Jimenez S, Álvarez-Hernán G, Diaz-Chamorro S, Barrera-Lopez J, Francisco-Morcillo J, Roman A, Centeno F, Carvajal-Gonzalez J
Frontiers in Cell and Developmental Biology 2021;9

nNOS regulates ciliated cell polarity, ciliary beat frequency, and directional flow in mouse trachea

Mikhailik A, Michurina T, Dikranian K, Hearn S, Maxakov V, Siller S, Takemaru K, Enikolopov G, Peunova N
Life Science Alliance 2021;4(5):e202000981

Planar cell polarity induces local microtubule bundling for coordinated ciliary beating

Nakayama S, Yano T, Namba T, Konishi S, Takagishi M, Herawati E, Nishida T, Imoto Y, Ishihara S, Takahashi M, Furuta K, Oiwa K, Tamura A, Tsukita S
Journal of Cell Biology 2021;220(7)

New mouse models for high resolution and live imaging of planar cell polarity proteins in vivo

Basta L, Hill-Oliva M, Paramore S, Sharan R, Goh A, Biswas A, Cortez M, Little K, Posfai E, Devenport D
Development 2021;148(18)

The molecular dynamics of subdistal appendages in multi-ciliated cells.

Ryu H, Lee H, Lee J, Noh H, Shin M, Kumar V, Hong S, Kim J, Park S
Nature communications 2021 Jan 27;12(1):612

A human ciliopathy reveals essential functions for NEK10 in airway mucociliary clearance

Chivukula R, Montoro D, Leung H, Yang J, Shamseldin H, Taylor M, Dougherty G, Zariwala M, Carson J, Daniels M, Sears P, Black K, Hariri L, Almogarri I, Frenkel E, Vinarsky V, Omran H, Knowles M, Tearney G, Alkuraya F, Sabatini D
Nature Medicine 2020;26(2):244-251

Multi-scale spatial heterogeneity enhances particle clearance in airway ciliary arrays

Ramirez-San Juan G, Mathijssen A, He M, Jan L, Marshall W, Prakash M
Nature Physics 2020;16(9):958-964

Non-canonical Wnt signalling regulates scarring in biliary disease via the planar cell polarity receptors

Wilson D, Jarman E, Mellin R, Wilson M, Waddell S, Tsokkou P, Younger N, Raven A, Bhalla S, Noll A, Olde Damink S, Schaap F, Chen P, Bates D, Banales J, Dean C, Henderson D, Sansom O, Kendall T, Boulter L
Nature Communications 2020;11(1)

Polarized cellular mechanoresponse system for maintaining radial size in developing epithelial tubes

Hirashima T, Adachi T
Development 2019

Domineering non-autonomy in Vangl1;Vangl2 double mutants demonstrates intercellular PCP signaling in the vertebrate inner ear

Stoller M, Roman O, Deans M
Developmental Biology 2018;437(1):17-26

Long-term preservation of planar cell polarity in reversed tracheal epithelium.

Tsuji T, Nakamura R, Katsuno T, Kishimoto Y, Suehiro A, Yamashita M, Uozumi R, Nakamura T, Tateya I, Omori K
Respiratory research 2018 Feb 2;19(1):22

Disruption of Core Planar Cell Polarity Signaling Regulates Renal Tubule Morphogenesis but Is Not Cystogenic

Kunimoto K, Bayly R, Vladar E, Vonderfecht T, Gallagher A, Axelrod J
Current Biology 2017;27(20):3120-3131.e4

Observing planar cell polarity in multiciliated mouse airway epithelial cells

Vladar E, Lee Y, Stearns T, Axelrod J
2015;127

Flattop regulates basal body docking and positioning in mono- and multiciliated cells

Gegg M, Böttcher A, Burtscher I, Hasenoeder S, Van Campenhout C, Aichler M, Walch A, Grant S, Lickert H
eLife 2014;3

Postnatal Refinement of Auditory Hair Cell Planar Polarity Deficits Occurs in the Absence of Vangl2

Copley C, Duncan J, Liu C, Cheng H, Deans M
Journal of Neuroscience 2013;33(35):14001-14016

Planar cell polarity effector gene Intu regulates cell fate-specific differentiation of keratinocytes through the primary cilia

Dai D, Li L, Huebner A, Zeng H, Guevara E, Claypool D, Liu A, Chen J
Cell Death & Differentiation 2012;20(1):130-138

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Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VANGL1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Sample type: Human
Protocol: Protocol

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Recombinant expression validation
Main image
Experimental details: Western blot analysis in control (vector only transfected HEK293T lysate) and VANGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403377).
Sample type: Human
Protocol: Protocol