Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-55231 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VANGL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DTESTYSGYS YYSSHSKKSH RQGERTRERH KSPRNKDGRG SEKSVTIQPP TGEPLLGNDS TRTEEVQDDN WGETTTAITG TSEHSISQED IARISKDMED SVGLDCKRY Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 95%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Deletions in VANGL1 are a risk factor for antibody-mediated kidney disease.
Jiang SH, Mercan S, Papa I, Moldovan M, Walters GD, Koina M, Fadia M, Stanley M, Lea-Henry T, Cook A, Ellyard J, McMorran B, Sundaram M, Thomson R, Canete PF, Hoy W, Hutton H, Srivastava M, McKeon K, de la RĂșa Figueroa I, Cervera R, Faria R, D'Alfonso S, Gatto M, Athanasopoulos V, Field M, Mathews J, Cho E, Andrews TD, Kitching AR, Cook MC, Riquelme MA, Bahlo M, Vinuesa CG
Cell reports. Medicine 2021 Dec 21;2(12):100475
Cell reports. Medicine 2021 Dec 21;2(12):100475
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VANGL1 in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a VANGL1 Polyclonal Antibody (Product # PA5-55231). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VANGL1 in control (vector only transfected HEK293T lysate) and VANGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a VANGL1 Polyclonal Antibody (Product # PA5-55231).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of VANGL1 in human cell line U-251 MG shows positivity in centrosome & nucleus but excluded from the nucleoli. Samples were probed using a VANGL1 Polyclonal Antibody (Product # PA5-55231).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of VANGL1 in MCF7 cells using VANGL1 Polyclonal Antibody (Product # PA5-55231) shows localization to plasma membrane.