MA5-24592

antibody from Invitrogen Antibodies

Targeting: VANGL1 STB2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24592

Provider

Invitrogen Antibodies

Product name

VANGL1 Monoclonal Antibody (CL0241)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: DTESTYSGYS YYSSHSKKSH RQGERTRERH KSPRNKDGRG SEKSVTIQPP TGEPLLGNDS TRTEEVQDDN WGETTTAITG TSEHSISQED IARISKDMED SVGLDCKRY

Antibody clone number

CL0241

Concentration

0.7 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using VANGL1 Monoclonal Antibody (CL0241) (Product # MA5-24592). Remaining relative intensity is presented. Loading control: Anti-PPIB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of VANGL1 in Lane1: Marker (kDa), Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: VANGL1 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403377). Samples were probed using a VANGL1 Monoclonal Antibody (Product # MA5-24592).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of VANGL1 in Lane1: Marker (kDa), Lane 2: Human breast cancer tissue cell line MCF7. Samples were probed using a VANGL1 Monoclonal Antibody (Product # MA5-24592).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using VANGL1 Monoclonal Antibody (CL0241) (Product # MA5-24592). Remaining relative intensity is presented. Loading control: Anti-PPIB.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of VANGL1 in MCF7 cells using the VANGL1 Monoclonal Antibody (CL0241) (Product # MA5-24592), showing specific staining of plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of VANGL1 in A-431 cells using the VANGL1 Monoclonal Antibody (CL0241) (Product # MA5-24592), showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of VANGL1 in MCF7 cells using the VANGL1 Monoclonal Antibody (CL0241) (Product # MA5-24592), showing specific staining of plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of VANGL1 in A-431 cells using the VANGL1 Monoclonal Antibody (CL0241) (Product # MA5-24592), showing specific staining of plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).