Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NBP1-86990 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86990, RRID:AB_11019907
- Product name
- Rabbit Polyclonal Vang-like Protein 1/VANGL1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Vang-like Protein 1/VANGL1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Observing planar cell polarity in multiciliated mouse airway epithelial cells.
Flattop regulates basal body docking and positioning in mono- and multiciliated cells.
Planar cell polarity effector gene Intu regulates cell fate-specific differentiation of keratinocytes through the primary cilia.
Postnatal refinement of auditory hair cell planar polarity deficits occurs in the absence of Vangl2.
Vladar EK, Lee YL, Stearns T, Axelrod JD
Methods in cell biology 2015;127:37-54
Methods in cell biology 2015;127:37-54
Flattop regulates basal body docking and positioning in mono- and multiciliated cells.
Gegg M, Böttcher A, Burtscher I, Hasenoeder S, Van Campenhout C, Aichler M, Walch A, Grant SG, Lickert H
eLife 2014 Oct 8;3
eLife 2014 Oct 8;3
Planar cell polarity effector gene Intu regulates cell fate-specific differentiation of keratinocytes through the primary cilia.
Dai D, Li L, Huebner A, Zeng H, Guevara E, Claypool DJ, Liu A, Chen J
Cell death and differentiation 2013 Jan;20(1):130-8
Cell death and differentiation 2013 Jan;20(1):130-8
Postnatal refinement of auditory hair cell planar polarity deficits occurs in the absence of Vangl2.
Copley CO, Duncan JS, Liu C, Cheng H, Deans MR
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Aug 28;33(35):14001-16
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Aug 28;33(35):14001-16
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Vang-like Protein 1/VANGL1 Antibody [NBP1-86990] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VANGL1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Vang-like Protein 1/VANGL1 Antibody [NBP1-86990] - Analysis in control (vector only transfected HEK293T lysate) and VANGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).