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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PA5-21706 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calsequestrin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HCT116. Predicted reactivity: Mouse (95%), Rat (96%), Dog (96%), Rabbit (96%), Chicken (90%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.68 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Calsequestrin 2 Polyclonal Antibody detects Calsequestrin 2 protein by western blot analysis. A. 30 µg HCT116 whole cell lysate/extract.10 % SDS-PAGE. Calsequestrin 2 Polyclonal Antibody (Product # PA5-21706) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Calsequestrin-2 antibody detects Calsequestrin-2 protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. Calsequestrin-2 antibody (Product # PA5-21706) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using Calsequestrin-2 (Product # PA5-21706) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
