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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- 712465 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HJURP Recombinant Polyclonal Antibody (16HCLC)
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- This antibody is predicted to react with Monkey, Pig, Cat.
- Antibody clone number
- 16HCLC
- Concentration
- 0.5 mg/mL
Submitted references HJURP regulates cell proliferation and chemo-resistance via YAP1/NDRG1 transcriptional axis in triple-negative breast cancer.
Mao M, Jia Y, Chen Y, Yang J, Xu L, Zhang X, Zhou J, Li Z, Chen C, Ju S, Wang L
Cell death & disease 2022 Apr 22;13(4):396
Cell death & disease 2022 Apr 22;13(4):396
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of HJURP was achieved by transfecting A549 cells with HJURP specific siRNA (Silencer® select Product # s30813 & s30814). Western blot analysis (Fig a) was performed using modified whole cell extracts (1% SDS) from HJURP knockdown cells (Lane 2) and non-specific scrambled siRNA transfected cells (Lane 1). The blot was probed with Anti-HJURP Recombinant Rabbit Polyclonal Antibody (Product # 712465, 1:500 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this Western blot is shown in the histogram (Fig b). Loss of signal upon siRNA mediated knockdown confirms that antibody is specific to HJURP.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (30 µg lysate) of HeLa (Lane 1), T98G (Lane 2), U-87 MG (Lane 3) and U-2 OS (Lane 4). The blot was probed with Anti-HJURP Recombinant Rabbit Polyclonal Antibody (Product # 712465, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). A ~83 kDa band corresponding to HJURP was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, A549 cells were fixed and permeabilized for detection of endogenous HJURP using Anti-HJURP Recombinant Rabbit Polyclonal Antibody (Product # 712465, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of HJURP protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of HJURP. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation-Western blot (ChIP-WB) of endogenous HJURP protein using Anti-HJURP Antibody: ChIP was performed using Anti-HJURP Recombinant Rabbit Polyclonal Antibody (Product # 712465, 10 µg) (Lane 3) on sheared chromatin from 4 million formaldehyde fixed A549 cells. Normal Rabbit IgG (lane 2) was used as negative IP control. Western blot analysis of immunoprecipitated proteins was performed using Anti-HJURP Recombinant Rabbit Polyclonal Antibody (Product # 712465, 1:200 dilution).
