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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- R32395 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD58 Antibody / LFA3
- Antibody type
- Polyclonal
- Antigen
- Amino acids F29-R215 from the human protein were used as the immunogen for the CD58 antibody.
- Description
- Antigen affinity
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the CD58 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa and 2) K562 cell lysate with CD58 antibody. Predicted molecular weight: 28/40-70 kDa (unmodified/glycosylated).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil with CD58 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HeLa cells with CD58 antibody at 1ug/10^6 cells (cells blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD58 antibody.
