H00000965-M02

antibody from Abnova Corporation

Targeting: CD58 LFA3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000965-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000965-M02, RRID:AB_10558758

Product name

CD58 monoclonal antibody (M02), clone 3B12-D5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant CD58.

Antigen sequence

MVAGSDAGRALGVLSVVCLLHCFGFISCFSQQIYG
VVYGNVTFHVPSNVPLKEVLWKKQKDKVAELENSE
FRAFSSFKNRVYLDTVSGSLTIYNLTSSDEDEYEM
ESPNITDTMKFFLYVLESLPSPTLTCALTNGSIEV
QCMIPEYYNSHRGLIMYSWDCPMEQCKRNSTSIYF
KMENDLPQKIQCTLSNPLFNTTSSIILTTCIPSSG
HSRHRYALIPIPLAVITTCIVLYMNGMYAF


Isotype

IgG

Antibody clone number

3B12-D5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CD58 expression in transfected 293T cell line by CD58 monoclonal antibody (M02), clone 3B12-D5.Lane 1: CD58 transfected lysate (Predicted MW: 28.1 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CD58 is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol