Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 17-0578-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD58 (LFA-3) Monoclonal Antibody (TS2/9), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The TS2/9 antibody recognizes human CD58, which is also known as Lymphocyte Associated Antigen-3 (LFA-3). CD58 exists both as a transmembrane protein as well as a GPI-linked extracellular protein. It has been shown that cells can express both forms concurrently. In addition, soluble CD58 has also been observed in serum, urine and synoval fluid of rheumatoid arthritis patients. CD58 is found on non-hematopoietic and most hematopoietic cells. Binding to its ligand CD2, which is present on T cells, initiates a signaling pathway which includes phosphorylation and results in release of cytokines. CD58 has been shown to be important in adhesion to various cell types, T cell activation, cytokine production and cytoxicity. The TS2/9 antibody has been reported to induce signaling events upon crosslinking. The epitope is in domain 1 of the immunoglobulin region. Applications Reported: This TS2/9 antibody has been reported for use in flow cytometric analysis. Applications Tested: This TS2/9 antibody has been pre-titrated and tested by flow cytometric analysis of normal peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- TS2/9
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Endoplasmic Reticulum (ER) Reorganization and Intracellular Retention of CD58 Are Functionally Independent Properties of the Human Cytomegalovirus ER-Resident Glycoprotein UL148.
Signal transduction by CD58: the transmembrane isoform transmits signals outside lipid rafts independently of the GPI-anchored isoform.
Amino acid residues required for binding of lymphocyte function-associated antigen 3 (CD58) to its counter-receptor CD2.
CD58 (LFA-3) stimulation provides a signal for human isotype switching and IgE production distinct from CD40.
Signal transduction pathways involved in T cell receptor-induced regulation of CD2 avidity for CD58.
Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
Nguyen CC, Domma AJ, Zhang H, Kamil JP
Journal of virology 2020 Feb 14;94(5)
Journal of virology 2020 Feb 14;94(5)
Signal transduction by CD58: the transmembrane isoform transmits signals outside lipid rafts independently of the GPI-anchored isoform.
Ariel O, Levi Y, Hollander N
Cellular signalling 2009 Jul;21(7):1100-8
Cellular signalling 2009 Jul;21(7):1100-8
Amino acid residues required for binding of lymphocyte function-associated antigen 3 (CD58) to its counter-receptor CD2.
Osborn L, Day ES, Miller GT, Karpusas M, Tizard R, Meuer SC, Hochman PS
The Journal of experimental medicine 1995 Jan 1;181(1):429-34
The Journal of experimental medicine 1995 Jan 1;181(1):429-34
CD58 (LFA-3) stimulation provides a signal for human isotype switching and IgE production distinct from CD40.
Diaz-Sanchez D, Chegini S, Zhang K, Saxon A
Journal of immunology (Baltimore, Md. : 1950) 1994 Jul 1;153(1):10-20
Journal of immunology (Baltimore, Md. : 1950) 1994 Jul 1;153(1):10-20
Signal transduction pathways involved in T cell receptor-induced regulation of CD2 avidity for CD58.
Hahn WC, Burakoff SJ, Bierer BE
Journal of immunology (Baltimore, Md. : 1950) 1993 Apr 1;150(7):2607-19
Journal of immunology (Baltimore, Md. : 1950) 1993 Apr 1;150(7):2607-19
Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
Sanchez-Madrid F, Krensky AM, Ware CF, Robbins E, Strominger JL, Burakoff SJ, Springer TA
Proceedings of the National Academy of Sciences of the United States of America 1982 Dec;79(23):7489-93
Proceedings of the National Academy of Sciences of the United States of America 1982 Dec;79(23):7489-93
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD58 (LFA-3) APC (purple histogram). Cells in the lymphocyte gate were used for analysis.