Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF1689 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD58/LFA-3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human CD58/LFA-3 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P19256
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Novel natural killer cell-mediated cancer immunotherapeutic activity of anisomycin against hepatocellular carcinoma cells.
Kim M, Lee SJ, Shin S, Park KS, Park SY, Lee CH
Scientific reports 2018 Jul 13;8(1):10668
Scientific reports 2018 Jul 13;8(1):10668
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD58/LFA-3 in Human Tonsil. CD58/LFA-3 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human CD58/LFA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1689) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of lymphocytes in lymphatic nodules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by CD58/LFA-3 and Neutral-ization by Human CD58/LFA-3 Antibody. Recombinant Human CD58/LFA-3 (Catalog # 1689-CD) stimulates proliferation in the human CD3+ T cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CD58/LFA-3 (0.2 µg/well) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD58/LFA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1689). The ND50 is typically 3-12 µg/mL in the presence of Mouse Anti-Human CD3 Monoclonal Antibody OKT3 (2 ng/well).