Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-1019-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD101 Monoclonal Antibody (BB27), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody BB27 reacts to human CD101 also known as V7 antigen, a member of the Ig superfamily. CD101 is a disulfide linked homodimer of unknown function. Expression of CD101 is found on monocytes, granulocytes and dendritic cells (Langerhan-like cells HLA-DR, CD1a, CD1c). In addition expression on T lymphocytes is important for cell activation. CD101+ CD28+ cells are very responsive to CD28 signaling. In combination with anti-CD28 or suboptimal levels of anti-CD3, anti-CD101 can increase proliferation thereby suggesting an activating role. The monoclonal antibody BB27 has been shown to inhibit the T cell reactivity in allogeneic and antigen-specific mixed DC-T cell cultures. Recently it has been demonstrated that mouse CD101 is found on a subpopulation of regulatory T cells (CD4, CD25, Foxp3 positive) that have high suppressor activity. Expression of CD101 on human PBMCs shows staining of about 30% of the Foxp3 positive cells. Studies have not confirmed higher suppressor activity in the human CD101 population. Applications Reported: This BB27 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining(frozen tissue). Applications Tested: This BB27 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BB27
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Comparative transcriptional and functional profiling defines conserved programs of intestinal DC differentiation in humans and mice.
Characterization of interleukin-17-producing regulatory T cells in inflamed intestinal mucosa from patients with inflammatory bowel diseases.
Differential proliferative responses in subsets of human CD28+ cells delineated by BB27 mAb.
Watchmaker PB, Lahl K, Lee M, Baumjohann D, Morton J, Kim SJ, Zeng R, Dent A, Ansel KM, Diamond B, Hadeiba H, Butcher EC
Nature immunology 2014 Jan;15(1):98-108
Nature immunology 2014 Jan;15(1):98-108
Characterization of interleukin-17-producing regulatory T cells in inflamed intestinal mucosa from patients with inflammatory bowel diseases.
Hovhannisyan Z, Treatman J, Littman DR, Mayer L
Gastroenterology 2011 Mar;140(3):957-65
Gastroenterology 2011 Mar;140(3):957-65
Differential proliferative responses in subsets of human CD28+ cells delineated by BB27 mAb.
Gouttefangeas C, Jacquot S, Meffre E, Schmid M, Boumsell L, Bensussan A
International immunology 1994 Mar;6(3):423-30
International immunology 1994 Mar;6(3):423-30
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.5 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (left) or 0.5 µg of Anti-Human CD101 Purified (right) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012) and subsequently with Anti-Human CD4 APC (Product # 17-0048-42). Cells in the lymphocyte gate were used for analysis.