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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- PA5-53602 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SP100 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: YKGFENVIHD KLPLQESEEE EREERSGLQL SLEQGTGENS FRSLTWPPSG SPSHAGTTPP ENGLSEHPCE TEQINAKRKD TTSDKDDSLG SQQTNEQCAQ KAEPTESCEQ IAVQVNNGDA GREMPCPL
- Concentration
- 0.1 mg/mL
Submitted references Lipid-associated PML structures assemble nuclear lipid droplets containing CCTα and Lipin1.
Lee J, Salsman J, Foster J, Dellaire G, Ridgway ND
Life science alliance 2020 Aug;3(8)
Life science alliance 2020 Aug;3(8)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SP100 in human cell line A-431 shows positivity in nucleus. Samples were probed using a SP100 Polyclonal Antibody (Product # PA5-53602).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SP100 in human cell line A-431 using a SP100 Polyclonal Antibody (Product # PA5-53602) shows localization to nucleus and nuclear bodies.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP100 in human lymph node using SP100 Polyclonal Antibody (Product # PA5-53602).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP100 in human colon using SP100 Polyclonal Antibody (Product # PA5-53602).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP100 in human testis using SP100 Polyclonal Antibody (Product # PA5-53602).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP100 in human spleen using SP100 Polyclonal Antibody (Product # PA5-53602).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP100 in human colon, lymph node, spleen and testis using SP100 Polyclonal Antibody (Product # PA5-53602) (A) shows similar protein distribution across tissues to an independent SP100 Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure S3. Lipid-associated PML structures are deficient in DAXX. (A, B) U2OS cells were untreated (panel A) or treated with 400 muM oleate for 24 h (panel B) before fixation and immunostaining for SP100 (red) and PML (green) and imaging by confocal microscopy. Lipid droplets were visualized with BODIPY 493/503 (blue) (bar, 10 mum). Images were captured as described in the legend to Fig 1 .
