Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [10]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-53476 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SP100 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QARTHHNQAS DIIVISSEDS EGSTDVDEPL EVFISAPRSE PVINNDNPLE SNDEKEGQEA TCSRPQIVPE PMDFRKLSTF RESFKKRVIG QDHDFSESSE Highest antigen sequence identity to the following orthologs: Mouse - 24%, Rat - 26%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SP100 in human cell lines SK-MEL-30 and HEK293 using a SP100 Polyclonal Antibody (Product # PA5-53476). Corresponding SP100 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP100 in human colon using SP100 Polyclonal Antibody (Product # PA5-53476).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP100 in human cerebral cortex using SP100 Polyclonal Antibody (Product # PA5-53476) shows no positivity in neurons as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP100 in human testis using SP100 Polyclonal Antibody (Product # PA5-53476).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP100 in human spleen using SP100 Polyclonal Antibody (Product # PA5-53476).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP100 in human lymph node using SP100 Polyclonal Antibody (Product # PA5-53476).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP100 in human tonsil and cerebral cortex tissues using SP100 Polyclonal Antibody (Product # PA5-53476). Corresponding SP100 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP100 in human colon, lymph node, spleen and testis using SP100 Polyclonal Antibody (Product # PA5-53476) (A) shows similar protein distribution across tissues to an independent SP100 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP100 in human small intestine using SP100 Polyclonal Antibody (Product # PA5-53476) shows moderate nuclear speckle positivity.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP100 in human placenta using SP100 Polyclonal Antibody (Product # PA5-53476) shows moderate nuclear speckle positivity.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP100 in human tonsil using SP100 Polyclonal Antibody (Product # PA5-53476) shows moderate nuclear speckle positivity.
