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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00006672-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006672-M02, RRID:AB_548318
- Product name
- Mouse Monoclonal SP100 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SP100 - SP100 nuclear antigen
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Quantification of the host response proteome after herpes simplex virus type 1 infection.
Berard AR, Coombs KM, Severini A
Journal of proteome research 2015 May 1;14(5):2121-42
Journal of proteome research 2015 May 1;14(5):2121-42
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SP100 Antibody (1G6) [H00006672-M02] - Analysis of SP100 over-expressed 293 cell line, cotransfected with SP100 Validated Chimera RNAi ( Cat # H00006672-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SP100 monoclonal antibody (M02), clone 1G6 (Cat # H00006672-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SP100 Antibody (1G6) [H00006672-M02] - Analysis of SP100 expression in transfected 293T cell line by SP100 monoclonal antibody (M02), clone 1G6.Lane 1: SP100 transfected lysate(100.417 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: SP100 Antibody (1G6) [H00006672-M02] - Detection limit for recombinant GST tagged SP100 is 0.03 ng/ml as a capture antibody.
