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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-78177 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SP100 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Jurkat, Raji, NCI-H929
- Concentration
- 0.76 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SP100 in whole cell lysate using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with SP100 polyclonal antibody (Product # PA5-78177) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SP100 Polyclonal Antibody (Product # PA5-78177). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP100 Polyclonal Antibody (Product # PA5-78177) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SP100 Polyclonal Antibody detects SP100 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SP100 protein stained by SP100 Polyclonal Antibody (Product # PA5-78177) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
