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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-85701 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SP100 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Positive Control: A431, HeLa, HepG2. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.6 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SP100 in various whole cell extracts using SP100 polyclonal antibody (Product # PA5-85701) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SP100 Polyclonal Antibody (Product # PA5-85701). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SP100 Polyclonal Antibody (Product # PA5-85701) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SP100 Polyclonal Antibody detects SP100 protein at P-body and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SP100 protein stained by SP100 Polyclonal Antibody (Product # PA5-85701) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
