NBP1-86060

antibody from Novus Biologicals

Targeting: SP100

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-86060

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-86060, RRID:AB_11032888

Product name

Rabbit Polyclonal SP100 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human SP100 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

The Smc5/6 Complex Restricts HBV when Localized to ND10 without Inducing an Innate Immune Response and Is Counteracted by the HBV X Protein Shortly after Infection.

Niu C, Livingston CM, Li L, Beran RK, Daffis S, Ramakrishnan D, Burdette D, Peiser L, Salas E, Ramos H, Yu M, Cheng G, Strubin M, Delaney WE IV, Fletcher SP
PloS one 2017;12(1):e0169648

---

Sp100 provides intrinsic immunity against human papillomavirus infection.

Stepp WH, Meyers JM, McBride AA
mBio 2013 Nov 5;4(6):e00845-13

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SP100 Antibody [NBP1-86060] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SP100 Antibody [NBP1-86060] - Analysis in human cell lines SK-MEL-30 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human gall bladder shows strong nuclear and cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human small intestine shows moderate nuclear speckle positivity.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human placenta shows moderate nuclear speckle positivity.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining in human tonsil and cerebral cortex tissues.. Corresponding SP100 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human tonsil shows moderate nuclear speckle positivity.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human spleen.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human cerebral cortex shows no positivity in neurons as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human colon.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human testis.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human lymph node.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human colon, lymph node, spleen and testis using Anti-SP100 antibody NBP1-86060 (A) shows similar protein distribution across tissues to independent antibody NBP1-89457 (B).