Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [11]
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Validation data
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- Product number
- NBP1-86060 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86060, RRID:AB_11032888
- Product name
- Rabbit Polyclonal SP100 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SP100 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The Smc5/6 Complex Restricts HBV when Localized to ND10 without Inducing an Innate Immune Response and Is Counteracted by the HBV X Protein Shortly after Infection.
Sp100 provides intrinsic immunity against human papillomavirus infection.
Niu C, Livingston CM, Li L, Beran RK, Daffis S, Ramakrishnan D, Burdette D, Peiser L, Salas E, Ramos H, Yu M, Cheng G, Strubin M, Delaney WE IV, Fletcher SP
PloS one 2017;12(1):e0169648
PloS one 2017;12(1):e0169648
Sp100 provides intrinsic immunity against human papillomavirus infection.
Stepp WH, Meyers JM, McBride AA
mBio 2013 Nov 5;4(6):e00845-13
mBio 2013 Nov 5;4(6):e00845-13
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SP100 Antibody [NBP1-86060] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SP100 Antibody [NBP1-86060] - Analysis in human cell lines SK-MEL-30 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human gall bladder shows strong nuclear and cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human small intestine shows moderate nuclear speckle positivity.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human placenta shows moderate nuclear speckle positivity.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining in human tonsil and cerebral cortex tissues.. Corresponding SP100 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human tonsil shows moderate nuclear speckle positivity.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human spleen.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human cerebral cortex shows no positivity in neurons as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human testis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human lymph node.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human colon, lymph node, spleen and testis using Anti-SP100 antibody NBP1-86060 (A) shows similar protein distribution across tissues to independent antibody NBP1-89457 (B).