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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- TA804575 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA804575, RRID:AB_2627357
- Product name
- POSTN mouse monoclonal antibody, clone OTI2B2 (formerly 2B2)
- Antibody type
- Monoclonal
- Description
- POSTN mouse monoclonal antibody, clone OTI2B2 (formerly 2B2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- POSTN
- Isotype
- IgG
- Antibody clone number
- OTI2B2
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Identification of novel biomarker candidates for immunohistochemical diagnosis to distinguish low-grade chondrosarcoma from enchondroma.
Lai X, Chen S
Proteomics 2015 Jul;15(13):2358-68
Proteomics 2015 Jul;15(13):2358-68
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POSTN (RC227934, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POSTN.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-POSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804575)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POSTN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804575)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunohistochemical staining for POSTN protein of negative control, positive control,enchondroma, and low grade chondrosarcoma (original magnification ?400) by using anti-POSTN antibody. Dilution: 1:1000
- Validation comment
- IHC
