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- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF2955 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Periostin/OSF-2 Isoform 2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Periostin/OSF-2 in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant rat Periostin/OSF-2 and recombinant human Periostin/OSF-2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q62009- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Periostin deletion suppresses late-phase response in mouse experimental allergic conjunctivitis.
New Noonan syndrome model mice with RIT1 mutation exhibit cardiac hypertrophy and susceptibility to β-adrenergic stimulation-induced cardiac fibrosis.
Multicolor quantitative confocal imaging cytometry.
Macrophage-Derived Granulin Drives Resistance to Immune Checkpoint Inhibition in Metastatic Pancreatic Cancer.
Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness.
Macrophage-secreted granulin supports pancreatic cancer metastasis by inducing liver fibrosis.
A mouse model for dominant collagen VI disorders: heterozygous deletion of Col6a3 Exon 16.
Periostin links mechanical strain to inflammation in abdominal aortic aneurysm.
Pressure overload-induced cardiac remodeling and dysfunction in the absence of interleukin 6 in mice.
Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts.
Periostin, a member of a novel family of vitamin K-dependent proteins, is expressed by mesenchymal stromal cells.
Asada Y, Okano M, Ishida W, Iwamoto S, Fukuda K, Hirakata T, Tada N, Fukushima A, Ebihara N, Kudo A, Matsuda A
Allergology international : official journal of the Japanese Society of Allergology 2019 Apr;68(2):233-239
Allergology international : official journal of the Japanese Society of Allergology 2019 Apr;68(2):233-239
New Noonan syndrome model mice with RIT1 mutation exhibit cardiac hypertrophy and susceptibility to β-adrenergic stimulation-induced cardiac fibrosis.
Takahara S, Inoue SI, Miyagawa-Tomita S, Matsuura K, Nakashima Y, Niihori T, Matsubara Y, Saiki Y, Aoki Y
EBioMedicine 2019 Apr;42:43-53
EBioMedicine 2019 Apr;42:43-53
Multicolor quantitative confocal imaging cytometry.
Coutu DL, Kokkaliaris KD, Kunz L, Schroeder T
Nature methods 2018 Jan;15(1):39-46
Nature methods 2018 Jan;15(1):39-46
Macrophage-Derived Granulin Drives Resistance to Immune Checkpoint Inhibition in Metastatic Pancreatic Cancer.
Quaranta V, Rainer C, Nielsen SR, Raymant ML, Ahmed MS, Engle DD, Taylor A, Murray T, Campbell F, Palmer DH, Tuveson DA, Mielgo A, Schmid MC
Cancer research 2018 Aug 1;78(15):4253-4269
Cancer research 2018 Aug 1;78(15):4253-4269
Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness.
Pryce BR, Al-Zahrani KN, Dufresne S, Belkina N, Labrèche C, Patino-Lopez G, Frenette J, Shaw S, Sabourin LA
Skeletal muscle 2017 Feb 2;7(1):3
Skeletal muscle 2017 Feb 2;7(1):3
Macrophage-secreted granulin supports pancreatic cancer metastasis by inducing liver fibrosis.
Nielsen SR, Quaranta V, Linford A, Emeagi P, Rainer C, Santos A, Ireland L, Sakai T, Sakai K, Kim YS, Engle D, Campbell F, Palmer D, Ko JH, Tuveson DA, Hirsch E, Mielgo A, Schmid MC
Nature cell biology 2016 May;18(5):549-60
Nature cell biology 2016 May;18(5):549-60
A mouse model for dominant collagen VI disorders: heterozygous deletion of Col6a3 Exon 16.
Pan TC, Zhang RZ, Arita M, Bogdanovich S, Adams SM, Gara SK, Wagener R, Khurana TS, Birk DE, Chu ML
The Journal of biological chemistry 2014 Apr 11;289(15):10293-307
The Journal of biological chemistry 2014 Apr 11;289(15):10293-307
Periostin links mechanical strain to inflammation in abdominal aortic aneurysm.
Yamashita O, Yoshimura K, Nagasawa A, Ueda K, Morikage N, Ikeda Y, Hamano K
PloS one 2013;8(11):e79753
PloS one 2013;8(11):e79753
Pressure overload-induced cardiac remodeling and dysfunction in the absence of interleukin 6 in mice.
Lai NC, Gao MH, Tang E, Tang R, Guo T, Dalton ND, Deng A, Tang T
Laboratory investigation; a journal of technical methods and pathology 2012 Nov;92(11):1518-26
Laboratory investigation; a journal of technical methods and pathology 2012 Nov;92(11):1518-26
Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts.
Xu BJ, Yan W, Jovanovic B, An AQ, Cheng N, Aakre ME, Yi Y, Eng J, Link AJ, Moses HL
Proteomics 2010 Jul;10(13):2458-70
Proteomics 2010 Jul;10(13):2458-70
Periostin, a member of a novel family of vitamin K-dependent proteins, is expressed by mesenchymal stromal cells.
Coutu DL, Wu JH, Monette A, Rivard GE, Blostein MD, Galipeau J
The Journal of biological chemistry 2008 Jun 27;283(26):17991-8001
The Journal of biological chemistry 2008 Jun 27;283(26):17991-8001
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Periostin/OSF-2 in Rat Mesenchymal Stem Cells. Periostin/OSF-2 was detected in immersion fixed rat mesenchymal stem cells differentiated to osteoblasts using Goat Anti-Mouse Periostin/OSF-2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Adhesion Mediated by Periostin/OSF-2 and Neutral-ization by Mouse Periostin/ OSF-2 Antibody. Recombinant Mouse Periostin/OSF-2 (Cata-log # 2955-F2), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recom-binant Mouse Periostin/OSF-2 (5 µg/mL) is neutralized (green line) by increasing concent-rations of Goat Anti-Mouse Periostin/ OSF-2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955). The ND50 is typically 1-5 µg/mL.
