AP07611SU-N

antibody from Acris Antibodies GmbH

Targeting: NOTCH2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP07611SU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP07611SU-N, RRID:AB_1620324

Product name

anti NOTCH2 (2396-2409)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to Amino acid residues 2396-2409 of Human Notch 2 (the total protein is 2471 aa).

Reactivity

Human

Host

Rabbit

Vial size

50 µl

Concentration

75 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using anti-Notch 2 (intra) antibody shows detection of a band at ~110 kD corresponding to active Notch 2 protein (arrowhead). Western Blot analysis was performed for Notch 2 expression using 100 µg of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1/400 dilution. ECL was used as visualization method.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Formalin-Fixed Paraffin-Embedded Human Kidney stained with NOTCH2 antibody Cat.-No AP07611SU-N