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- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP07611SU-N - Provider product page
- Provider
- OriGene
- Product name
- NOTCH2 (2396-2409) rabbit polyclonal antibody, Serum
- Antibody type
- Polyclonal
- Description
- NOTCH2 (2396-2409) rabbit polyclonal antibody, Serum
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- NOTCH2
- Antibody clone number
- NULL
- Vial size
- 50 µl
- Concentration
- 75 mg/ml
Submitted references Differential expression of Notch receptors and their ligands in desmoplastic ameloblastoma.
Siar CH, Nakano K, Han PP, Nagatsuka H, Ng KH, Kawakami T
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2010 Aug 1;39(7):552-8
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2010 Aug 1;39(7):552-8
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot using anti-Notch 2 (intra) antibody shows detection of a band at ~110 kD corresponding to active Notch 2 protein (arrowhead). Western Blot analysis was performed for Notch 2 expression using 100 ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1/400 dilution. ECL was used as visualization method.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-Fixed Paraffin-Embedded Human Kidney stained with NOTCH2 antibody
- Validation comment
- IHC
