PA5-27458

antibody from Invitrogen Antibodies

Targeting: NOTCH2

Western blot (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

PA5-27458

Provider

Invitrogen Antibodies

Product name

NOTCH2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, HeLa, HepG2. Predicted reactivity: Mouse (95%), Rat (89%), Rhesus Monkey (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.43 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Notch 2 using 30 µg of HeLa lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a Notch 2 polyclonal antibody (Product # PA5-27458) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of NOTCH2 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NOTCH2 Polyclonal Antibody (Product # PA5-27458) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NOTCH2 Polyclonal Antibody (Product # PA5-27458). Sample (30 µg of whole cell lysate). Lane A:NIH-3T3 5% SDS PAGE. NOTCH2 Polyclonal Antibody (Product # PA5-27458) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-NOTCH2 Polyclonal Antibody (Product # PA5-27458) and a 300 kDa band corresponding to NOTCH2 was observed in HaCaT. Whole cell extracts (40 µg lysate) of HaCaT (Lane 1), SH-SY5Y (Lane 2), HeLa (Lane 3), U-87 MG (Lane 4) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).