R1559

antibody from Acris Antibodies GmbH

Targeting: NOTCH2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R1559

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1559, RRID:AB_1005753

Product name

anti NOTCH2 (Intra)

Antibody type

Polyclonal

Antigen

A synthetic peptide corresponding to amino acid residues 2396-2409 of Human Notch 2 (the total protein is 2471 aa). A residue of cysteine was added to the amino terminal end to facilitate coupling.

Reactivity

Human, Mouse, Rat, Canine

Host

Rabbit

Vial size

0.2 ml

Concentration

75 mg/ml (by Refractometry)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using R1559 Notch2 antibody (intra) shows detection of a band at ~110 kDa corresponding to active Notch 2 protein (arrowhead). Western Blot analysis was performed for Notch2 expression using 100μg of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2.This band is not seen in the control cell. The ntracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1/400 dilution. ECL was used as visualization method.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

R1559 NOTCH2 antibody stainng of Formalin Fixed, Paraffin Embedded Human kidney tissue at 1/500 dilution. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.