ANR-071-200UL

antibody from Invitrogen Antibodies

Targeting: NMUR1 FM-3, GPC-R, GPR66, NMU1R

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ANR-071-200UL

Provider

Invitrogen Antibodies

Product name

NMUR1 (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1-2. Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1:500). 3-4. Anti-NMUR1 (extracellular) Antibody , preincubated with NMUR1 (extracellular) Blocking Peptide (BLP-NR071).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human HT-29 colon adenocarcinoma cell line lysate (lanes 1 and 5), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 6), human K562 myelogenous leukemia cell line lysate (lanes 3 and 7) and human NK-92 natural killer cell line lysate (lanes 4 and 8): - 1-4. Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1:400). 5-8. Anti-NMUR1 (extracellular) Antibody , preincubated with NMUR1 (extracellular) Blocking Peptide (BLP-NR071).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of NMUR1 in rat cerebellum. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1: 300), followed by goat Anti-rabbit-AlexaFluor-488. A. NMUR1 immunoreactivity (green) appears in neuron profiles (arrows). B. Pre-incubation of the Antibody with NMUR1 (extracellular) Blocking Peptide (BLP-NR071), suppressed staining. Cell nuclei are stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of NMUR1 in rat cerebellum. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NMUR1 (extracellular) Antibody (#ANR-071), (1: 300), followed by goat Anti-rabbit-AlexaFluor-488. A. NMUR1 immunoreactivity (green) appears in neuron profiles (arrows). B. Pre-incubation of the Antibody with NMUR1 (extracellular) Blocking Peptide (BLP-NR071), suppressed staining. Cell nuclei are stained with DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of NMUR1 by indirect flow cytometry in live intact mouse J774 macrophage cell line: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-NMUR1 (extracellular) Antibody (#ANR-071), (2.5μg) + goat- Anti-rabbit-FITC.