Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C59247 - Provider product page

- Provider
- LSBio
- Product name
- ALPI / Alkaline Phosphatase Antibody LS-C59247
- Antibody type
- Polyclonal
- Description
- Delipidated and defibrinated
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Store at 4°C.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image

- Experimental details
- Anti-Alkaline Phosphatase Antibody - Western Blot. Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (LS-C60884) diluted 1:4000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.