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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP1480c - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP1480c, RRID:AB_2227108
- Product name
- Alkaline Phosphatase (ALPI) Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Growth differentiation factor 9 concentration in the follicular fluid of infertile women with endometriosis.
Hendarto H, Prabowo P, Moeloek FA, Soetjipto S
Fertility and sterility 2010 Jul;94(2):758-60
Fertility and sterility 2010 Jul;94(2):758-60
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Western blot analysis of lysate from human placenta tissue,using ALPI Antibody (Center)(Cat. #AP1480c).AP1480c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane."
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Fluorescent confocal image of HeLa cells stained with Alkaline Phosphatase (ALPI) (Center) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1480c Alkaline Phosphatase (ALPI) (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). Alkaline Phosphatase (ALPI) immunoreactivity is localized to the cytoplasm of HeLa cells."
- Primary Ab dilution
- 1:100
