LS-B10745

antibody from LSBio

Targeting: ALPI IAP

Western blot (Enhanced validation)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-B10745

Provider

LSBio

Product name

IHC-plus™ ALPI / Alkaline Phosphatase Antibody (aa397-458, clone 3B10E7) LS-B10745

Antibody type

Monoclonal

Description

Protein G purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3B10E7

Storage

Short term: store at 4°C. Long term: store at -20°C.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot using ALPI monoclonal antibody against human ALPI recombinant protein. (Expected MW is 31.9 kDa)

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot using ALPI monoclonal antibody against HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot using ALPI mouse monoclonal antibody against HL60 (1) and HepG2 (2) cell lysate.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Black: Control Antigen (100ng); Purple: Antigen (10ng); Blue: Antigen (50ng); Red: Antigen (100ng);

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Black: Control Antigen (100ng); Purple: Antigen (10ng); Blue: Antigen (50ng); Red: Antigen (100ng);

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Black: Control Antigen (100ng); Purple: Antigen (10ng); Blue: Antigen (50ng); Red: Antigen (100ng);

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-ALPI / Alkaline Phosphatase antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-ALPI / Alkaline Phosphatase antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-ALPI / Alkaline Phosphatase antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-ALPI / Alkaline Phosphatase antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.