AP07542PU-N

antibody from Acris Antibodies GmbH

Targeting: ALPI IAP

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP07542PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP07542PU-N, RRID:AB_1611414

Product name

anti Alkaline phosphatase / ALPI / IAP

Antibody type

Polyclonal

Antigen

Purified Alkaline Phosphatase from Human intestine

Reactivity

Human

Host

Rabbit

Vial size

50 µl

Concentration

10.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot. Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1/5000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] diluted 1/4000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Formalin-Fixed Paraffin-Embedded Human Small intestine stained with Alkaline Phosphatase Antibody Cat.-No AP07542PU-N at 5 µg/ml after heat-induced antigen retrieval.