Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP07542PU-N - Provider product page
- Provider
- OriGene
- Product name
- Alkaline Phosphatase (ALPI) rabbit polyclonal antibody, Purified
- Antibody type
- Polyclonal
- Description
- Alkaline Phosphatase (ALPI) rabbit polyclonal antibody, Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ALPI
- Antibody clone number
- NULL
- Vial size
- 50 µl
- Concentration
- 10 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot. Both the antiserum and IgG fractions of?anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions?of SDS-PAGE the 60000 dalton enzyme in cellular extracts. Approximately 10 ug of total?protein is loaded per lane. A 1/5000 dilution of the primary antibody is used followed by?detection using HRP Goat-a-Rabbit IgG [H&L] diluted 1/4000 and color?development using 4-CN substrate until sufficient color develops. Other detection systems will?yield similar results.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-Fixed Paraffin-Embedded Human Small intestine stained with Alkaline Phosphatase Antibody at 5 ug/ml after heat-induced antigen retrieval.
- Validation comment
- IHC