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- Antibody Data
- Antigen structure
- References [2]
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- Western blot [1]
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- Product number
- NB600-588 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-588, RRID:AB_10003075
- Product name
- Rabbit Polyclonal Alkaline Phosphatase, Intestinal Antibody
- Antibody type
- Polyclonal
- Description
- Delipidation and Defibrination. This polyclonal reacts with alkaline phosphatase from human intestine showing a 60 kD band against this enzyme under reducing conditions of SDS-PAGE. Elevated expression of this enzyme is associated with undifferentiated pluripoten stem cell (PSC).
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.2 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Mybl2, downregulated during colon epithelial cell maturation, is suppressed by miR-365.
Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer.
Papetti M, Augenlicht LH
American journal of physiology. Gastrointestinal and liver physiology 2011 Sep;301(3):G508-18
American journal of physiology. Gastrointestinal and liver physiology 2011 Sep;301(3):G508-18
Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer.
Wilson AJ, Byun DS, Popova N, Murray LB, L'Italien K, Sowa Y, Arango D, Velcich A, Augenlicht LH, Mariadason JM
The Journal of biological chemistry 2006 May 12;281(19):13548-58
The Journal of biological chemistry 2006 May 12;281(19):13548-58
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB600-588] - Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops.
