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Other assayAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Other assay [2]
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- Product number
- PA5-22210 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Intestinal Alkaline Phosphatase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (87%), Rat (87%), Bovine (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.16 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Human ALPI deficiency causes inflammatory bowel disease and highlights a key mechanism of gut homeostasis.
Mechanisms Involved in the Development of the Chronic Gastrointestinal Syndrome in Nonhuman Primates after Total-Body Irradiation with Bone Marrow Shielding.
Parlato M, Charbit-Henrion F, Pan J, Romano C, Duclaux-Loras R, Le Du MH, Warner N, Francalanci P, Bruneau J, Bras M, Zarhrate M, Bègue B, Guegan N, Rakotobe S, Kapel N, De Angelis P, Griffiths AM, Fiedler K, Crowley E, Ruemmele F, Muise AM, Cerf-Bensussan N
EMBO molecular medicine 2018 Apr;10(4)
EMBO molecular medicine 2018 Apr;10(4)
Mechanisms Involved in the Development of the Chronic Gastrointestinal Syndrome in Nonhuman Primates after Total-Body Irradiation with Bone Marrow Shielding.
Shea-Donohue T, Fasano A, Zhao A, Notari L, Yan S, Sun R, Bohl JA, Desai N, Tudor G, Morimoto M, Booth C, Bennett A, Farese AM, MacVittie TJ
Radiation research 2016 Jun;185(6):591-603
Radiation research 2016 Jun;185(6):591-603
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Reduced ALPI expression in patients' small intestinal biopsies A, B Immunofluorescence microscopy of duodenum (A) and ileum (B) sections from Patient 1 (A) and Patient 2 (B) compared to normal or IBD controls. Sections were stained with DAPI (4',6-diamidino-2-phenylindole) for DNA (blue) and antibodies against ALPI alone (second column, red) or ALPI and E-cadherin (green) (first column, merge staining). Magnification 20x (scale bar = 100 mum). C Effect of AP inhibitors (L-phe, L-arg) on AP activity in stools of non-inflamed controls ( n = 20). Data are expressed as mean +- SD. **** P < 0.0001, nonparametric, unpaired two-tailed Mann-Whitney test. D ALPI activity in stools of non-inflamed ( n = 20) or IBD controls ( n = 14) and of P1. AP values are expressed as units of ALPI/g stool and as medians with interquartiles; * P = 0.0122, nonparametric, unpaired two-tailed Mann-Whitney test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure EV4 TNAP expression in patients' small intestinal biopsies A, B Immunofluorescence microscopy of duodenum (A) and ileum (B) sections from P1 (A) and P2 (B) compared with normal or IBD controls. Sections were counterstained with blue RedDot2 dye for DNA and antibodies against ALPI alone (red), TNAP alone (green) or TNAP and ALPI (first column, merge staining). Magnification 20x (Scale bars: 100 mum).
