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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- GTX82524 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX82524, RRID:AB_11179164
- Product name
- EIF4E2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Distinct recruitment of human eIF4E isoforms to processing bodies and stress granules.
An oxygen-regulated switch in the protein synthesis machinery.
Frydryskova K, Masek T, Borcin K, Mrvova S, Venturi V, Pospisek M
BMC molecular biology 2016 Aug 30;17(1):21
BMC molecular biology 2016 Aug 30;17(1):21
An oxygen-regulated switch in the protein synthesis machinery.
Uniacke J, Holterman CE, Lachance G, Franovic A, Jacob MD, Fabian MR, Payette J, Holcik M, Pause A, Lee S
Nature 2012 May 6;486(7401):126-9
Nature 2012 May 6;486(7401):126-9
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4E2 Antibody (GTX82524) in NCI-H460 cell line lysates (35μg/lane). EIF4E2 (arrow) was detected using the purified Pab.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4E2 (arrow) using rabbit polyclonal EIF4E2 Antibody (GTX82524). 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4E2 gene.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E2 Antibody(GTX82524), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
