PA5-20953

antibody from Invitrogen Antibodies

Targeting: PIAS3 FLJ14651, ZMIZ5

Provider product page for PA5-20953

Western blot

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [2]
Comments [0]
Validations
Other assay [2]

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Product number: PA5-20953 - Provider product page
Provider: Invitrogen Antibodies
Product name: PIAS3 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: A suggested positive control is K562 cell lysate. PA5-20953 can be used with blocking peptide PEP-1067.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

PIAS3 protein structure - PA5-20953 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 IL-6 or ox-LDL stimulation reduces PIAS3 expression in RAW264.7 cells. ( A ) IL-6 treatment leads to reductions in PIAS3 expression levels in a time- and dose-dependent manner, as determined via RT-qPCR. ( B ) Detection of PIAS3, STAT3-Y705 and STAT3 protein expression in IL-6-treated cells via Western blotting. Full-length blots were presented in Supplementary Figure S2 . ( C ) Detection of IL-6 and PIAS3 expression in ox-LDL-stimulated cells via RT-qPCR. ( D ) Stimulation with ox-LDL reduces PIAS3 protein expression. PIAS3, STAT3-Y705, STAT3 and iNOS protein expression levels in ox-LDL-treated cells were detected via Western blotting. Full-length blots were presented in Supplementary Figure S3 . ( E ) Relative levels of NO in the plasma of ApoE -/- mice fed a 20-week chow or western diet. Fold changes in NO levels relative to NO levels in mice fed a chow diet are shown (n = 8). Regarding the RT-qPCR results, relative transcript levels are expressed as fold changes compared to transcript levels in mock-treated cells. Regarding the Western blotting results, the relative protein levels in treated cells compared with those in mock-treated cells are shown below the images. *P < 0.05, **P < 0.01, ***P < 0.001.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Atherosclerotic plaque formation is inversely correlated with PIAS3 expression in the aorta. ( A ) Representative images of Oil Red O-stained aortas. ( B ) Representative images showing atherosclerotic plaque formation at the aortic root (bar, 200 mum). ( C ) The relative sizes of the plaque areas at the aortic roots are expressed as fold changes relative to the sizes of the plaque areas of ApoE -/- mice fed a chow diet (n = 8). Serial frozen sections of aortic roots were subjected to Oil Red O staining, and plaque areas were assessed. ( D ) IL-6 and PIAS3 gene expression levels in aortic tissue were determined by RT-qPCR (n = 8). ( E ) PIAS3, STAT3-Y705, STAT3 and IkappaBalpha protein expression levels in aortic tissue were determined by Western blotting. Full-length blots were presented in Supplementary Figure S1 . ( F ) Densitometry analysis of the Western blotting results in panel E. The average protein levels after normalization with beta-actin are expressed as fold changes relative to the protein levels in WT mice fed a western diet. ( G ) Representative images of PIAS3 protein detected in macrophages (Mphi) in atherosclerotic lesions. Serial frozen sections of aortic roots were subjected to immunohistochemical staining with Moma2 (for staining Mphi) and PIAS3 antibodies. Oil Red O staining was also performed to visualize the plaques (bar, 100 mum). *P < 0.05, **P < 0.01, ***P < 0.001.