Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA328652 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-TRPM8 (extracellular)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-TRPM8 (extracellular)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- TRPM8
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of prostate carcinoma cell lines; DU145 (lanes 1,3), LNCaP (lanes 2,4) and mouse-TRPM8 transfected HEK-293 (lanes 5,6) cell lysates : 1,2,5. Anti-TRPM8 (extracellular) antibody, (1:200). 3,4,6. Anti-TRPM8 (extracellular) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of TRPM8 in LNCaP prostate carcinoma cell line. Immunocytochemical staining of LNCaP cells with Anti-TRPM8 (extracellular) antibody. A. Extracellular staining of intact LNCaP cells with Anti-TRPM8 (extracellular) antibody (1:100), followed by goat anti-rabbit-AlexaFluor-550 (red), (x100). B. Intracellular staining of LNCaP cells with Anti-TRPM8 (extracellular) antibody (1:1000), followed by goat anti-rabbit-AlexaFluor-488 (green), (x100).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemical staining of intact living rat root dorsal root ganglion (DRG) cells. A. Intracellular staining of cells with Anti-TRPM8 (extracellular) antibody, (1:500) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Extracellular staining of live cells with Anti-TRPM8 (extracellular) antibody (1:50) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. The cell-permeable dye Hoechst 33342 (blue) was used for nuclear staining.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of TRPM8 in rat DRG. Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPM8 (extracellular) antibody, (1:100). TRPM8 is expressed in DRG neurons. Hoechst 33342 is used as the counterstain.
- Validation comment
- IHC
