Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- M01002 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-TRPM8 Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Rabbit IgG Monoclonal antibody for TRPM8 detection. Tested positive for WB, ICC/IF in Human;Mouse.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Antibody clone number
- ADBI-20
- Vial size
- 100ug
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- IF analysis of TRPM8 using anti- TRPM8 antibody (M01002) TRPM8 was detected in immunocytochemical section of LnCaP cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- TRPM8 Antibody (M01002) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
- Additional image