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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-96613 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBE2Z Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PPPGMFVVPD TVDMTKIHAL ITGPFDTPYE GGFFLFVFRC PPDYPIHPPR VKLMTTGNNT VRFNPNFYRN GKVCLSILGT WTGPAWSPAQ SISSVLISIQ SLMTENPYHN EPGFEQERHP GDSKNYNECI RHETIRVAVC DMMEGKCPCP EPLRGVMEKS FLEYYDFYEV ACKDRLHLQG QTMQDPFGEK RGHFDYQSLL MRLGLIRQKV LERLHNENAE MDSDSSSSGT ETDLHGSLRV; Positive Samples: BT-474, MCF7, HepG2, HeLa, Mouse pancreas, Mouse testis; Cellular Location: Cytoplasm, Nucleus
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.43 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of UBE2Z in extracts of various cell lines using UBE2Z Polyclonal Antibody (Product # PA5-96613) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of UBE2Z was performed in A-549 cells using UBE2Z Polyclonal Antibody (Product # PA5-96613).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of UBE2Z in paraffin-embedded human kidney using UBE2Z Polyclonal Antibody (Product # PA5-96613) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of UBE2Z in paraffin-embedded mouse kidney using UBE2Z Polyclonal Antibody (Product # PA5-96613) at a dilution of 1:200.
