PA5-96613

antibody from Invitrogen Antibodies

Targeting: UBE2Z FLJ13855, USE1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96613

Provider

Invitrogen Antibodies

Product name

UBE2Z Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PPPGMFVVPD TVDMTKIHAL ITGPFDTPYE GGFFLFVFRC PPDYPIHPPR VKLMTTGNNT VRFNPNFYRN GKVCLSILGT WTGPAWSPAQ SISSVLISIQ SLMTENPYHN EPGFEQERHP GDSKNYNECI RHETIRVAVC DMMEGKCPCP EPLRGVMEKS FLEYYDFYEV ACKDRLHLQG QTMQDPFGEK RGHFDYQSLL MRLGLIRQKV LERLHNENAE MDSDSSSSGT ETDLHGSLRV; Positive Samples: BT-474, MCF7, HepG2, HeLa, Mouse pancreas, Mouse testis; Cellular Location: Cytoplasm, Nucleus

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.43 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of UBE2Z in extracts of various cell lines using UBE2Z Polyclonal Antibody (Product # PA5-96613) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of UBE2Z was performed in A-549 cells using UBE2Z Polyclonal Antibody (Product # PA5-96613).