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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunohistochemistry [1]
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- Product number
- NBP1-82245 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-82245, RRID:AB_11020011
- Product name
- Rabbit Polyclonal UBE2Z Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat UBE2Z antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: UBE2Z Antibody [NBP1-82245] - Simple Western lane view shows a specific band for UBE2Z in 0.2 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: UBE2Z Antibody [NBP1-82245] - Electropherogram image(s) of corresponding Simple Western lane view. UBE2Z antibody was used at 1:20 dilution on NIH-3T3 lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE2Z Antibody [NBP1-82245] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE2Z Antibody [NBP1-82245] - Analysis in human cell line A-549.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE2Z Antibody [NBP1-82245] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBE2Z Antibody [NBP1-82245] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts and moderate in Leydig cells.
