MA5-55905

antibody from Invitrogen Antibodies

Targeting: UBE2Z FLJ13855, USE1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-55905

Provider

Invitrogen Antibodies

Product name

UBE2Z Monoclonal Antibody (K52034_14C7)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: WDGERTAPQC LLRIKRDIMS IYKEPPPGMF VVPDTVDMTK IHALITGPFD TPYEGGFFLF VFRCPPDYPI HPPRVKLMTT GNNTVRFNPN FYRNGKVCLS ILGTWTGPAW SPAQSISSVL ISIQSLMTEN PYHNEPGFEQ ERHPGDSKNY NECIRHETIR VAVCDMMEGK CPCPEPLRGV MEKSFLEYYD FYEVACKDRL HLQGQTMQDP FGEKRGHFDY QSLLMRLGLI RQKVLERLHN ENAEMDSDSS

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

K52034_14C7

Vial size

50 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of UBE2Z in 15 µg of MCF-7. Sample was run on 6-18% SDS-PAGE under reducing conditions, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. UBE2Z band was visualized using ECL Western Blotting Substrate. Incubation with primary UBE2Z monoclonal antibody (Product # MA5-55905) at a dilution of 1 µg/mL was used.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of UBE2Z in 200 µg of MCF7 lysate. Samples are as follows: Lane 1: MCF7 lysate, Lane 2: UBE2Z immunoprecipitated from MCF7 lysate, Lane3: The same as Lane 2 but KT82 was used as IgG isotype control antibody. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Incubation of samples with UBE2Z monoclonal antibody (Product # MA5-55905) at a dilution of 2.5 µg was used.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of UBE2Z in paraffin-embedded testis tissue. Sample was incubated with UBE2Z monoclonal antibody (Product # MA5-55905) at a dilution of 5 µg/mL (RT, 1 hour). Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.