Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-77984 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GBX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: U87-MG, SK-N-SH, IMR32, IMR32 nuclear, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, mouse fetal brain, rat brain Predicted Reactivity: Bovine (96%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.41 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GBX2 in mouse tissue extract using 50 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with GBX2 polyclonal antibody (Product # PA5-77984) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GBX2 in rat tissue extract using 50 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with GBX2 polyclonal antibody (Product # PA5-77984) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GBX2 Polyclonal Antibody detects GBX2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 Polyclonal Antibody (Product # PA5-77984) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GBX2 Polyclonal Antibody (Product # PA5-77984). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 Polyclonal Antibody (Product # PA5-77984) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GBX2 Polyclonal Antibody (Product # PA5-77984). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 Polyclonal Antibody (Product # PA5-77984) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GBX2 Polyclonal Antibody (Product # PA5-77984). Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 Polyclonal Antibody (Product # PA5-77984) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GBX2 Polyclonal Antibody detects GBX2 protein by western blot analysis. IMR32 whole cell extracts and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 Polyclonal Antibody (Product # PA5-77984) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GBX2 Polyclonal Antibody detects GBX2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 Polyclonal Antibody (Product # PA5-77984) diluted by 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GBX2 Polyclonal Antibody detects GBX2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GBX2 protein stained by GBX2 Polyclonal Antibody (Product # PA5-77984) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
