Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NB100-55393 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-55393, RRID:AB_838288
- Product name
- Goat Polyclonal GBX2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Goat
- Antigen sequence
QGKDESKVEDDPK
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Elongation factor 1 alpha1 and genes associated with Usher syndromes are downstream targets of GBX2.
Evolutionarily conserved function of Gbx2 in anterior hindbrain development.
Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency.
Roeseler DA, Sachdev S, Buckley DM, Joshi T, Wu DK, Xu D, Hannink M, Waters ST
PloS one 2012;7(11):e47366
PloS one 2012;7(11):e47366
Evolutionarily conserved function of Gbx2 in anterior hindbrain development.
Burroughs-Garcia J, Sittaramane V, Chandrasekhar A, Waters ST
Developmental dynamics : an official publication of the American Association of Anatomists 2011 Apr;240(4):828-38
Developmental dynamics : an official publication of the American Association of Anatomists 2011 Apr;240(4):828-38
Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency.
Palmqvist L, Glover CH, Hsu L, Lu M, Bossen B, Piret JM, Humphries RK, Helgason CD
Stem cells (Dayton, Ohio) 2005 May;23(5):663-80
Stem cells (Dayton, Ohio) 2005 May;23(5):663-80
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GBX2 Antibody [NB100-55393] - (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393] - Gbx2 Antibody [NB100-55393] - (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393] - (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393] - Staining of paraffin embedded Human Placenta at dilution of 5ug/ml. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393] - Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393] - Staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.