Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF4638 - Provider product page
- Provider
- R&D Systems
- Product name
- Human GBX2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human GBX2 in Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P52951
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Generation of thalamic neurons from mouse embryonic stem cells.
Shiraishi A, Muguruma K, Sasai Y
Development (Cambridge, England) 2017 Apr 1;144(7):1211-1220
Development (Cambridge, England) 2017 Apr 1;144(7):1211-1220
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human GBX2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human GBX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GBX2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.