AF2074

antibody from Novus Biologicals

Targeting: FCGR1A CD64, CD64A

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2074

Provider

Novus Biologicals

Product name

Goat Polyclonal Fc gamma RI/CD64 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse Fc gamma RI/CD64 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human Fc gamma RI is observed and less than 5% cross-reactivity with recombinant mouse (rm) Fc gamma RIIB and rmFc gamma RIII is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse Fc gamma RI/CD64 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Fc gamma RI/CD64 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2074) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Fc gamma RI/CD64 at approximately 60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.