MAB2074

antibody from Novus Biologicals

Targeting: FCGR1A CD64, CD64A

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2074

Provider

Novus Biologicals

Product name

Rat Monoclonal Fc gamma RI/CD64 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse Fc gamma RI/CD64 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Fc gamma RIIA or rhFc gamma RIIIB is observed.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Systemic inflammation modulates Fc receptor expression on microglia during chronic neurodegeneration.

Lunnon K, Teeling JL, Tutt AL, Cragg MS, Glennie MJ, Perry VH
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):7215-24

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse Fc gamma RI/CD64 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Fc gamma RI/CD64 Monoclonal Antibody (Catalog # MAB2074) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Fc gamma RI/CD64 at approximately 60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.