TA506343

antibody from OriGene

Targeting: FCGR1A CD64, CD64A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

TA506343

Provider

OriGene

Proper citation

OriGene Cat#TA506343, RRID:AB_2623718

Product name

FCGR1A (CD64) mouse monoclonal antibody, clone OTI3D3 (formerly 3D3)

Antibody type

Monoclonal

Description

FCGR1A (CD64) mouse monoclonal antibody, clone OTI3D3 (formerly 3D3)

Host

Mouse

Conjugate

Unconjugated

Epitope

FCGR1A

Isotype

IgG

Antibody clone number

OTI3D3

Vial size

100 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR1A (RC207487, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR1A.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-FCGR1A monoclonal antibody at 1:200 dilution.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-FCGR1A mouse monoclonal antibody (TA506343) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR1A(RC207487).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FCGR1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506343)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FCGR1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506343)

Validation comment

IHC