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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB2074 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Fc gamma RI/CD64 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Fc gamma RI/CD64 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Fc gamma RIIA or rhFc gamma RIIIB is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P26151- Isotype
- IgG
- Antibody clone number
- 290305
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Systemic inflammation modulates Fc receptor expression on microglia during chronic neurodegeneration.
Lunnon K, Teeling JL, Tutt AL, Cragg MS, Glennie MJ, Perry VH
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):7215-24
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):7215-24
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse Fc gamma RI/CD64 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Fc gamma RI/CD64 Monoclonal Antibody (Catalog # MAB2074) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Fc gamma RI/CD64 at approximately 60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
