Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- RQ7170 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD64 Antibody / FCGR1A
- Antibody type
- Polyclonal
- Description
- This highly specific CD64 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the CD64 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human ThP-1, 2) rat spleen, 3) rat thymus, 4) rat PC-3, 5) mouse EL-4, 6) mouse spleen, 7) mouse thymus, 8) mouse RAW264.7 and 9) mouse ANA-1 cell lysate with CD64 antibody. Predicted molecular weight: 39-75 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human SiHa cells with CD64 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD64 antibody.