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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB6173 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6173, RRID:AB_1577828
- Product name
- Mlph polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of Mlph.
- Antigen sequence
C-ARHIFAKPVMAQQP- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A coiled-coil domain of melanophilin is essential for Myosin Va recruitment and melanosome transport in melanocytes.
Hume AN, Tarafder AK, Ramalho JS, Sviderskaya EV, Seabra MC
Molecular biology of the cell 2006 Nov;17(11):4720-35
Molecular biology of the cell 2006 Nov;17(11):4720-35
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The Mlph polyclonal antibody (Cat # PAB6173) staining (0.5ug/mL) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may be the stacking-spearating gel interface. Data kindly provided by A N Hume, Imperial College.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Wild-type C57bl6 derived melanocytes were stained with the Mlph polyclonal antibody (Cat # PAB6173) (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.
- Validation comment
- Immunocytochemistry
