LS-B10383

antibody from LSBio

Targeting: SF3B4 Hsh49, SAP49, SF3b49

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B10383

Provider

LSBio

Product name

IHC-plus™ SF3B4 Antibody (clone 3A1) LS-B10383

Antibody type

Monoclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat, Bovine, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

3A1

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Human HeLa cells stained with SF3B4 antibody (red), chicken antibody to vimentin (green) and DNA (blue, stained with DAPI). The SF3B4 antibody antibody reveals strong granular nuclear staining which is a little different from the DNA stain and presumably reflects splicosomal complexes. The vimentin antibody stains the cytoplasmic intermediate filament network of the HeLa cells.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Human HeLa cells stained with SF3B4 antibody (red), chicken antibody to vimentin (green) and DNA (blue, stained with DAPI). The SF3B4 antibody antibody reveals strong granular nuclear staining which is a little different from the DNA stain and presumably reflects splicosomal complexes. The vimentin antibody stains the cytoplasmic intermediate filament network of the HeLa cells.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Human HeLa cells stained with SF3B4 antibody (red), chicken antibody to vimentin (green) and DNA (blue, stained with DAPI). The SF3B4 antibody antibody reveals strong granular nuclear staining which is a little different from the DNA stain and presumably reflects splicosomal complexes. The vimentin antibody stains the cytoplasmic intermediate filament network of the HeLa cells.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-SF3B4 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.