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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-28441 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-SF3B4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLa, NIH-3T3, JC. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.59 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Sf3b4-depleted Xenopus embryos: A model to study the pathogenesis of craniofacial defects in Nager syndrome.
Devotta A, Juraver-Geslin H, Gonzalez JA, Hong CS, Saint-Jeannet JP
Developmental biology 2016 Jul 15;415(2):371-382
Developmental biology 2016 Jul 15;415(2):371-382
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SF3B4 using 30 µg of A) NIH-3T3 and B) JC lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a SF3B4 polyclonal antibody (Product # PA5-28441) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SF3B4 using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a SF3B4 polyclonal antibody (Product # PA5-28441) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SF3B4 in paraformaldehyde-fixed HeLa cells using a SF3B4 polyclonal antibody (Product # PA5-28441) at a 1:200 dilution.
